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Recombinant CHO-K1 cells expressing the cell surface glycoprotein TROP2 (Trophoblast cell-surface antigen 2); ref. seq. NM_002353.2.
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Our frozen, irradiated cAMPZen cells express a variety of GPCRs for binding and functional assays. No cell culture is required prior to testing.
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Our frozen, irradiated AequoZen cells express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light.
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Image Search Results
Journal: Journal of Biological Chemistry
Article Title: Nicotinic Acid Receptor Agonists Differentially Activate Downstream Effectors
doi: 10.1074/jbc.m701866200
Figure Lengend Snippet: FIGURE 1. Structures of GPR109A agonists presented herein with compound designations indicated below each structure.
Article Snippet: Measurement of Adenylyl Cyclase Inhibition A 96-well Adenylyl Cyclase Activation Flashplate AssayTM kit (
Techniques:
Journal: Journal of Biological Chemistry
Article Title: Nicotinic Acid Receptor Agonists Differentially Activate Downstream Effectors
doi: 10.1074/jbc.m701866200
Figure Lengend Snippet: FIGURE6.MouseflushingviaLaserDoppler.Base-lineperfusionwasestablishedfor3minintheventralright ear of C57Bl/6 mice. Mice were then given intraperitoneal injections of individual GPR109A agonists. Values are the mean % change from base-line, preinjection values S.E. of 3–26 individual animals as indicated in the key at right.
Article Snippet: Measurement of Adenylyl Cyclase Inhibition A 96-well Adenylyl Cyclase Activation Flashplate AssayTM kit (
Techniques:
Journal: Journal of Biological Chemistry
Article Title: Nicotinic Acid Receptor Agonists Differentially Activate Downstream Effectors
doi: 10.1074/jbc.m701866200
Figure Lengend Snippet: FIGURE 9. Qualitative assessment of GPR109A internalization following agonist exposure. The dose- dependent internalization of GPR109A was examined using immunofluorescence microscopy on tran- siently transfected COS-7 cells following 30 min of exposure to the indicated doses of nicotinic acid (left), compound 1a (middle), or compound 4a (right). Data shown are representative of at least three independ- ent experiments.
Article Snippet: Measurement of Adenylyl Cyclase Inhibition A 96-well Adenylyl Cyclase Activation Flashplate AssayTM kit (
Techniques: Immunofluorescence, Microscopy, Transfection
Journal: Journal of Biological Chemistry
Article Title: Nicotinic Acid Receptor Agonists Differentially Activate Downstream Effectors
doi: 10.1074/jbc.m701866200
Figure Lengend Snippet: FIGURE 10. Qualitative assessment of GPR109A internalization following 10 M agonist exposure for 30 min. Internalization of GPR109A was examined using immunofluorescence microscopy on transiently trans- fected COS-7 cells following 30 min of exposure to 10 M of indicated compounds. Data shown are represent- ative of at least three independent experiments.
Article Snippet: Measurement of Adenylyl Cyclase Inhibition A 96-well Adenylyl Cyclase Activation Flashplate AssayTM kit (
Techniques: Immunofluorescence, Microscopy